Article
Functional characterization of the somatic hypermutation process leading to antibody D1.3, a high affinity antibody directed against lysozyme.
Journal of immunology (Baltimore, Md. : 1950) - 15 Feb 1999
England P, Nageotte R, Renard M, Page A L, Bedouelle H
Abstract excerpt
The impact of somatic hypermutation on the affinity of Abs directed against protein Ags remains poorly understood. We chose as a model the secondary response Ab D1.3 directed against hen egg lysozyme. During the maturation process leading to this Ab, five replacement somatic mutations occurred. A...
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Affinity
- Base Sequence
- Chickens
- Immunoglobulin Variable Region
- Kinetics
- Mice
- Models, Biological
- Models, Molecular
- Molecular Sequence Data
- Muramidase
- Mutagenesis, Site-Directed
- Mutation