Article
Sequence-based typing of HLA class II DQB1.
Tissue antigens - 1 Apr 2000
Dinauer D M, Luhm R A, Uzgiris A J, Eckels D D, Hessner M J
Abstract excerpt
Due to the expanding number of known HLA class II DQB1 alleles, high-resolution oligotyping is becoming ineffective, therefore a sequence-based typing (SBT) strategy was developed to provide rapid and definitive typing of HLA-DQB1. HLA-DQB1*02, *03, *04, *05, and *06 alleles were individually amplified by polymerase chain reaction (PCR) using exon 2 group-specific primers. Forward and reverse PCR primers were...
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