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Article

Sequencing of HLA class II genes based on the conserved diversity of the non-coding regions: sequencing based typing of HLA-DRB genes.

Tissue antigens - 1 May 1999

Kotsch K, Wehling J, Blasczyk R

Abstract excerpt

In this paper, we present a novel sequencing based typing strategy for the HLA-DRB1, 3, 4 and 5 loci. The new approach is based on a group-specific amplification from intron 1 to intron 2 according to the serologically-defined antigens. For this purpose, we have determined the 3' 500 bp-fragment of intron 1 and the 5' 340 bp-fragment of intron 2 of all serological antigens and their most frequent subtypes. We...

Topics

Alleles
Base Sequence
Conserved Sequence
Genetic Variation
HLA-DR Antigens
Histocompatibility Testing
Humans
Introns
Molecular Sequence Data
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 10372544
DOI: 10.1034/j.1399-0039.1999.530505.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-10372544-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Sequencing of HLA class II genes based on the conserved diversity of the non-coding regions: sequencing based typing of HLA-DRB genes.PMID 10372544Read ↗

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