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Article

An improved integration replacement/disruption method for mutagenesis of yeast essential genes.

Genes & genetic systems - 1 Feb 2000

Tohe A, Oguchi T

Abstract excerpt

We improved the integration replacement/disruption method (Shortle, D., Novic, P., and Botstein, D. Proc. Natl. Acad. Sci. USA 81: 4889-4893, 1984) for isolating mutants in any of essential genes of the yeast Saccharomyces cerevisiae by integrating mutagenized DNA into the wild type gene of interest. We adopted this method to isolate temperature-sensitive mutants of the MPC1 gene encoding the YLL031C ORF. To...

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Source

Identifiers

PMID: 10846619
DOI: 10.1266/ggs.75.33

Genes

MPC1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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