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Gene disruption and basic phenotypic analysis of nine novel yeast genes from chromosome XIV.

Yeast (Chichester, England) - 15 Sept 2000

Capozzo C, Sartorello F, Dal Pero F, D'Angelo M, Vezzi A, Campanaro S, Valle G

Abstract excerpt

In this work, we describe the disruption of nine ORFs of S. cerevisiae (YNL123w, YNL119w, YNL115c, YNL108c, YNL110c, YNL124w, YNL233w, YNL232w and YNL231c) in two genetic backgrounds: FY1679 and CEN.PK2. For the construction of the deletant strains, we used the strategy of short flanking homology (SFH) PCR. The SFH-deletion cassette was made by PCR amplification of the KanMX4 module with primers containing a 5'...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 10953080
DOI: 10.1002/1097-0061(20000915)16:12<1089::AID-YEA600>3.0.CO;2-G

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Source receipt: pubmed, retrieved 7 Sept 2026.

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