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Article

Disruption and basic phenotypic analysis of 18 novel genes from the yeast Saccharomyces cerevisiae.

Yeast (Chichester, England) - 30 Jan 1999

Wysocki R, Roganti T, Van Dyck E, de Kerchove D'Exaerde A, Foury F

Abstract excerpt

In the frame of the European Network for Functional Analysis (EUROFAN) we have deleted 18 yeast open reading frames (ORFs) from chromosomes II, X and XIV using the short flanking homology-PCR strategy. Two diploid strains were used: FY1679 and CEN.PK2. The deletion kanMX6 cassettes with long flanking homology and the cognate gene clones have also been constructed. Heterozygous diploid deletant strains have been...

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Identifiers

PMID: 10029995
DOI: 10.1002/(SICI)1097-0061(19990130)15:2<165::AID-YEA351>3.0.CO;2-V

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Source receipt: pubmed, retrieved 7 Sept 2026.

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