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Article

Functional expression of human DNA topoisomerase I and its subcellular localization in HeLa cells.

Experimental cell research - 1 May 2000

Mo Y Y, Wang P, Beck W T

Abstract excerpt

DNA topoisomerase (topo) I plays an important role in DNA metabolism by relieving the torsional restraints of DNA topology through ATP-independent single-strand DNA breakage. In the present study, we expressed human topo I in HeLa cells by fusing it to enhanced green fluorescent protein (EGFP). The EGFP-topo I fusion protein is functionally active in that it relaxes supercoiled plasmid DNA; forms complexes with...

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Identifiers

PMID: 10772820
DOI: 10.1006/excr.2000.4864

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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