Article
Determination of a cAMP-dependent protein kinase phosphorylation site in the C-terminal region of human endothelial actin-binding protein.
Archives of biochemistry and biophysics - 1 May 2000
Jay D, García E J, Lara J E, Medina M A, de la Luz Ibarra M
Abstract excerpt
Three different C-terminal regions of human endothelial actin-binding protein-280 (ABP-280 or ABP; nonmuscle filamin) were subcloned and efficiently expressed in the Escherichia coli BL21 (DE3) system as indicated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. As predicted by the aminoacid sequence one of the fragments, a 109-kDa peptide (residues 1671-2647), contained a calpain cleavage site and...
Topics
- Amino Acid Motifs
- Amino Acid Substitution
- Blood Platelets
- Calpain
- Contractile Proteins
- Cyclic AMP-Dependent Protein Kinases
- Endothelium
- Filamins
- Humans
- Immune Sera
- Microfilament Proteins
- Molecular Weight
- Mutation
- Peptide Fragments