Article
Transformation of Aspergillus giganteus to hygromycin B resistance.
Current genetics - 1 Jan 1990
Wnendt S, Jacobs M, Stahl U
Abstract excerpt
A wild strain of A. giganteus was transformed to hygromycin B resistance using a bacterial resistance gene under the control of A. nidulans sequences. Stable transformants arose by heterogenous integration, mainly of tandem repeats of vector DNA at various sites in the host genome. Between 6 and 30 resistant colonies were obtained per microgram DNA per 3 x 10(3) viable protoplasts. Vector DNA could be recovered...
Topics
- Anti-Bacterial Agents
- Aspergillus
- Blotting, Southern
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Genes, Fungal
- Genetic Vectors
- Hygromycin B
- Phenotype
- Transformation, Bacterial
