Article
Transformation of the cultivated mushroom, Agaricus bisporus, to hygromycin B resistance.
Molecular & general genetics : MGG - 25 Feb 1996
van de Rhee M D, Graça P M, Huizing H J, Mooibroek H
Abstract excerpt
Application of biotechnology to the cultivated mushroom, Agaricus bisporus, has been hampered thus far by the lack of a transformation system. Here, transformation of both a homo- and a heterokaryotic strain of A. bisporus to hygromycin B resistance is described. Transforming DNA was integrated into the A. bisporus genome and stably maintained throughout vegetative growth. Transformants of the heterokaryotic...
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