Article
Transformation of the cultivated mushroom, Agaricus bisporus, to hygromycin B resistance.
Molecular & general genetics : MGG - 25 Feb 1996
van de Rhee M D, Graça P M, Huizing H J, Mooibroek H
Abstract excerpt
Application of biotechnology to the cultivated mushroom, Agaricus bisporus, has been hampered thus far by the lack of a transformation system. Here, transformation of both a homo- and a heterokaryotic strain of A. bisporus to hygromycin B resistance is described. Transforming DNA was integrated i...
Topics
- Agaricus
- Anti-Bacterial Agents
- Aspergillus nidulans
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- DNA Primers
- Drug Resistance, Microbial
- Gene Expression Regulation, Fungal
- Glyceraldehyde-3-Phosphate Dehydrogenases
- Hygromycin B
