Article
Detection of beryllium sensitivity using a flow cytometric lymphocyte proliferation test: the Immuno-Be-LPT.
Toxicology - 21 Feb 2000
Farris G M, Newman L S, Frome E L, Shou Y, Barker E, Habbersett R C, Maier L, Smith H N, Marrone B L
Abstract excerpt
Measurement of lymphocyte proliferation to detect hypersensitivity to beryllium (Be-LPT) in vitro is done presently using a method based on tritiated thymidine incorporation. Although this method is sensitive it gives no information on cell viability or responding lymphocyte subsets. We have developed reliable and simple flow cytometric assays for lymphocyte proliferation testing (Immuno-Be-LPT) by combining...
Topics
- Antibodies, Monoclonal
- Antigens, CD
- Antigens, Differentiation, T-Lymphocyte
- Beryllium
- Biomarkers
- Bromodeoxyuridine
- Cell Division
- Chronic Disease
- DNA
- Flow Cytometry
- Fluorescent Dyes
- Humans
- Lectins, C-Type
- Light