Article
Toxicity, mutation frequency and mutation spectrum induced by dacarbazine in CHO cells expressing different levels of O(6)-methylguanine-DNA methyltransferase.
Mutation research - 14 Feb 2000
Psaroudi M C, Kyrtopoulos S A
Abstract excerpt
The toxicity and mutagenicity (including the mutation spectrum induced) of dacarbazine, a methylating cytostatic drug, was examined in CHO cells expressing different levels of the repair enzyme O(6)-methylguanine-DNA methyltransferase (MGMT). Expression of low or high levels of a transfected human MGMT gene under the control of the metallothionein promoter protected the cells against dacarbazine-induced toxicity...
Topics
- Amino Acid Substitution
- Animals
- CHO Cells
- Cricetinae
- DNA Methylation
- DNA Mutational Analysis
- Dacarbazine
- Enzyme Induction
- Guanine
- Humans
- Hypoxanthine Phosphoribosyltransferase
- Liver Extracts
- Male
- Methylnitrosourea
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase
- Point Mutation
- Rats