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Article

Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger.

Microbiology (Reading, England) - 1 Feb 2000

Gordon Caroline L, Khalaj Vahid, Ram Arthur F J, Archer David B, Brookman Jayne L, Trinci Anthony P J, Jeenes David J, Doonan John H, Wells Brian, Punt Peter J, van den Hondel Cees A M J J, Robson Geoffrey D

Abstract excerpt

A glucoamylase::green fluorescent protein fusion (GLA::sGFP) was constructed which allows the green fluorescent protein to be used as an in vivo reporter of protein secretion in Aspergillus niger. Two secretory fusions were designed for secretion of GLA::sGFP which employed slightly different lengths of the glucoamylase protein (GLA499 and GLA514). Expression of GLA::sGFP revealed that fluorescence was localized...

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PMID: 10708380
DOI: 10.1099/00221287-146-2-415

Genes

GLA

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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