Article
Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation.
Acta biochimica Polonica - 1 Jan 2005
Perlińska-Lenart Urszula, Kurzatkowski Wiesław, Janas Piotr, Kopińska Agnieszka, Palamarczyk Grazyna, Kruszewska Joanna S
Abstract excerpt
O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated...
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