Article
Fluorescence based assay of GAL system in yeast Saccharomyces cerevisiae.
FEMS microbiology letters - 1 Mar 2005
Stagoj Mateja Novak, Comino Aleksandra, Komel Radovan
Abstract excerpt
The GAL1 promoter is one of the strongest inducible promoters in the yeast Saccharomyces cerevisiae. In order to improve recombinant protein production we have developed a fluorescence based method for screening and evaluating the contribution of various gene deletions to protein expression from the GAL1 promoter. The level of protein synthesis was determined in 28 selected mutant strains simultaneously, by...
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