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Article

Cytochemical evaluation of localization and secretion of a heterologous enzyme displayed on yeast cell surface.

FEMS microbiology letters - 15 Nov 2000

Shibasaki Y, Kamasawa N, Shibasaki S, Zou W, Murai T, Ueda M, Tanaka A, Osumi M

Abstract excerpt

A starch-utilizing Saccharomyces cerevisiae strain was constructed by cell surface engineering. Distribution of the heterologous glucoamylase-alpha-agglutinin fusion protein on the yeast cell was analyzed by indirect fluorescence microscopy using an anti-glucoamylase antibody. Most of the intense fluorescence was first localized in the small bud, then observed on the entire cell wall of the daughter and mother...

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PMID: 11064202
DOI: 10.1111/j.1574-6968.2000.tb09389.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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