Article
Late onset of CAD gene amplification in unamplified PALA resistant Chinese hamster mutants.
Cancer letters - 31 Mar 2000
Mucciolo E, Bertoni L, Mondello C, Giulotto E
Abstract excerpt
In rodent cells, resistance to PALA (N-phosphonacetyl-L-aspartate) has always been found associated with amplification of the CAD gene (carbamyl-P synthetase, aspartate transcarbamylase, dihydro-orotase). We describe two PALA resistant Chinese hamster mutant cell lines in which amplification of the CAD gene was not present. The PALA resistant phenotype was stable when the cells were grown in non-selective medium....
Topics
- Animals
- Antineoplastic Agents
- Aspartate Carbamoyltransferase
- Aspartic Acid
- CHO Cells
- Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
- Cricetinae
- Dihydroorotase
- Drug Resistance, Neoplasm
- Gene Amplification
- Gene Dosage
- Multienzyme Complexes
- Mutation
- Phosphonoacetic Acid