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Article

Different roles for abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene.

Nucleic acids research - 15 Mar 2000

Lascaris R F, Groot E, Hoen P B, Mager W H, Planta R J

Abstract excerpt

In vivo mutational analysis of the yeast RPS28A ribosomal protein (rp-)gene promoter demonstrated that both the Abf1p binding site and the adjacent T-rich element are essential for efficient transcription. In vivo Mnase and DNaseI digestion showed that the RPS28A promoter contains a 50-60 bp long nucleosome-free region directly downstream from the Abf1p binding site, followed by an ordered array of nucleosomes....

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Identifiers

PMID: 10684934
DOI: 10.1093/nar/28.6.1390
PMCID: PMC111049

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