Article
Different roles for abf1p and a T-rich promoter element in nucleosome organization of the yeast RPS28A gene.
Nucleic acids research - 15 Mar 2000
Lascaris R F, Groot E, Hoen P B, Mager W H, Planta R J
Abstract excerpt
In vivo mutational analysis of the yeast RPS28A ribosomal protein (rp-)gene promoter demonstrated that both the Abf1p binding site and the adjacent T-rich element are essential for efficient transcription. In vivo Mnase and DNaseI digestion showed that the RPS28A promoter contains a 50-60 bp long nucleosome-free region directly downstream from the Abf1p binding site, followed by an ordered array of nucleosomes....
Topics
- Base Sequence
- Binding Sites
- DNA Footprinting
- DNA-Binding Proteins
- Deoxyribonuclease I
- Dopamine Plasma Membrane Transport Proteins
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Membrane Glycoproteins
- Membrane Transport Proteins
- Micrococcal Nuclease
- Molecular Conformation