Article
Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair.
The Journal of biological chemistry - 25 Feb 2000
Feaver W J, Huang W, Gileadi O, Myers L, Gustafsson C M, Kornberg R D, Friedberg E C
Abstract excerpt
A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast...
Topics
- Adenosine Triphosphatases
- Caffeine
- DNA Helicases
- DNA Repair
- Fungal Proteins
- Genetic Complementation Test
- Models, Biological
- Mutation
- Phosphodiesterase Inhibitors
- Plasmids
- Saccharomyces cerevisiae Proteins
- TATA-Binding Protein Associated Factors