Article
Complex interactions between yeast TFIIIB and TFIIIC.
The Journal of biological chemistry - 23 Jun 1995
Chaussivert N, Conesa C, Shaaban S, Sentenac A
Abstract excerpt
Transcription of yeast class III genes requires the sequential assembly of the general transcription factors TFIIIC and TFIIIB, and of RNA polymerase III, into an initiation complex composed of at least 25 polypeptides. The 70-kDa subunit of TFIIIB (TFIIIB70) is central in this network of interactions as it contacts both TATA-binding protein and a subunit of polymerase III. We show here that the TATA-binding...
Topics
- Cloning, Molecular
- DNA-Binding Proteins
- Escherichia coli
- Fungal Proteins
- Genes, Fungal
- Genetic Variation
- Histidine
- Macromolecular Substances
- Mutagenesis, Site-Directed
- Point Mutation
- RNA Polymerase III