Rapid detection of efflux pumps and their relation with drug resistance in yeast cells.

doi:10.1002/(sici)1097-0320(20000101)39:1<26::aid-cyto5>3.0.co;2-c

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Article

Rapid detection of efflux pumps and their relation with drug resistance in yeast cells.

Cytometry - 1 Jan 2000

Prudêncio C, Sansonetty F, Sousa M J, Côrte-Real M, Leão C

Abstract excerpt

BACKGROUND: Cell drug resistance can be due to the presence of active efflux pumps (AEP). Identification of yeast cells with a resistance phenotype is important either from a clinical, agricultural or biotechnological point of view. Rapid and reliable methods to detect AEP can be therefore very useful. METHODS: Some yeast cells change their staining by calcein-AM, BCECF-AM, rhodamine 123 and DiOC(5), when...

Topics

ATP-Binding Cassette Transporters
Benomyl
Biological Transport, Active
Cycloheximide
Drug Resistance
Flow Cytometry
Fungicides, Industrial
Microbial Sensitivity Tests
Microscopy, Fluorescence
Phenotype
Saccharomyces cerevisiae
Schizosaccharomyces
Triazoles

Source

Open in PubMed

Identifiers

PMID: 10655560
DOI: 10.1002/(sici)1097-0320(20000101)39:1<26::aid-cyto5>3.0.co;2-c

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