Article
Analysis of the p53 tumor suppressor gene by pyrosequencing.
BioTechniques - 1 Jan 2000
Ahmadian A, Lundeberg J, Nyrén P, Uhlén M, Ronaghi M
Abstract excerpt
Tumor suppressor genes are implicated in cell cycle progression. Inactivation of these genes predominantly occurs through mutations and/or allelic loss that involves both alleles. With inactivation by multiple mutations in a single gene, cloning of the amplified gene is necessary to determine whether the mutations reside on one or both alleles. Using pyrosequencing, a recently developed approach based on...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.