Article
Barriers to heterologous expression of a selenoprotein gene in bacteria.
Journal of bacteriology - 1 Feb 1997
Tormay P, Böck A
Abstract excerpt
The specificity parameters counteracting the heterologous expression in Escherichia coli of the Desulfomicrobium baculatum gene (hydV) coding for the large subunit of the periplasmic hydrogenase which is a selenoprotein have been studied. hydV'-'lacZ fusions were constructed, and it was shown that they do not direct the incorporation of selenocysteine in E. coli. Rather, the UGA codon is efficiently suppressed by...
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