Rapid cycle allele-specific amplification: studies with the cystic fibrosis delta F508 locus.

Article

Rapid cycle allele-specific amplification: studies with the cystic fibrosis delta F508 locus.

Clinical chemistry - 1 May 1993

Wittwer C T, Marshall B C, Reed G H, Cherry J L

Abstract excerpt

Rapid cycle DNA amplification is a polymerase chain reaction technique with improved product specificity and cycle times of 20-60 s, allowing complete 30-cycle reactions in 10-30 min. The presence or absence of the delta F508 deletion and wild-type allele was determined in 104 cystic fibrosis pat...

Topics

Base Sequence
Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
Genotype
Heterozygote
Homozygote
Humans
Membrane Proteins
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Temperature
Time Factors

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