Article
Effective expression and purification of recombinant onconase, an antitumor protein.
FEBS letters - 17 Dec 1999
Notomista E, Cafaro V, Fusiello R, Bracale A, D'Alessio G, Di Donato A
Abstract excerpt
Several members of the RNase A superfamily are endowed with antitumor activity, showing selective cytotoxicity toward several tumor cell lines. One of these is onconase, the smallest member of the RNase A superfamily, which is at present undergoing phase III clinical trials. We report here the expression of recombinant onconase in Escherichia coli inclusion bodies, the correct processing of the protein, followed...
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