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Article

Elastase substrate specificity tailored through substrate-assisted catalysis and phage display.

Protein engineering - 1 Nov 1999

Dall'Acqua W, Halin C, Rodrigues M L, Carter P

Abstract excerpt

The catalytic histidine of human neutrophil elastase was replaced with alanine (H57A) to determine if a substrate histidine could substitute for the missing catalytic group-'substrate-assisted catalysis'. H57A and wild-type elastase were recovered directly from Pichia pastoris following expression from a synthetic gene lacking the elastase pro sequence, thereby obviating the need for zymogen activation. Potential...

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Identifiers

PMID: 10585504
DOI: 10.1093/protein/12.11.981

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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