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Article

Construct for high-level expression and low misincorporation of lysine for arginine during expression of pET-encoded eukaryotic proteins in Escherichia coli.

BioTechniques - 1 Nov 1999

You J, Cohen R E, Pickart C M

Abstract excerpt

The arginine codon AGA is rarely used in E. coli but is common in eukaryotic genes. Prior studies have shown that the low level of tRNA(UCUArg) can lead to low expression and misincorporation of lysine for arginine, during expression of genes containing AGA codons in E. coli. The chloramphenicol-selectable plasmid pJY2 is designed to facilitate the expression of such genes cloned into pET vectors: it encodes T7...

Topics

Arginine
Bacterial Proteins
Biopolymers
Escherichia coli
Gene Expression Regulation, Bacterial
Lysine
Mass Spectrometry
Mutation
Plasmids
Polyubiquitin
Protein Biosynthesis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Transfer, Arg

Recombinant Proteins

Ubiquitins

Source

Identifiers

PMID: 10572642
DOI: 10.2144/99275st01

Genes

AGA

Provenance

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