Article
Initiation of protein synthesis from a termination codon.
Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1990
Varshney U, RajBhandary U L
Abstract excerpt
We show that the amber termination codon UAG can initiate protein synthesis in Escherichia coli. We mutated the initiation codon AUG of the chloramphenicol acetyltransferase (CAT) gene to UAG (CATam1) and translated mRNA derived from the mutant CAT gene in E. coli S-30 extracts. A full-length CAT...
Topics
- Anti-Bacterial Agents
- Base Sequence
- Chloramphenicol O-Acetyltransferase
- Cloning, Molecular
- Codon
- Drug Resistance, Microbial
- Escherichia coli
- Genes, Bacterial
- Genes, Regulator
- Genetic Vectors
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Biosynthesis
- RNA, Messenger
- RNA, Transfer
- RNA, Transfer, Amino Acyl
- Terminator Regions, Genetic