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Article

Cysteine misincorporation in bacterially expressed human α‐synuclein

24 Feb 2006

Abstract excerpt

Bacterially expressed human alpha-synuclein (alpha-syn) has been widely used in structural and functional studies. Here we show that approximately 20% of human alpha-syn expressed in Escherichia coli is mistranslated and that a Cys residue is incorporated at position 136 instead of a Tyr. Site-directed mutagenesis of codon 136 (TAC to TAT) resulted in the expression of alpha-syn lacking Cys. Although wild-type...

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Identifiers

PMID: 16513114
DOI: 10.1016/j.febslet.2006.02.032

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Source receipt: pubmed, retrieved 7 Sept 2026.

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