Article
Replisome assembly at oriC, the replication origin of E. coli, reveals an explanation for initiation sites outside an origin.
Molecular cell - 1 Oct 1999
Fang L, Davey M J, O'Donnell M
Abstract excerpt
This study outlines the events downstream of origin unwinding by DnaA, leading to assembly of two replication forks at the E. coli origin, oriC. We show that two hexamers of DnaB assemble onto the opposing strands of the resulting bubble, expanding it further, yet helicase action is not required. Primase cannot act until the helicases move 65 nucleotides or more. Once primers are formed, two molecules of the...
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