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Disruption and functional analysis of six ORFs on chromosome IV: YDR013w, YDR014w, YDR015c, YDR018c, YDR020c, YDR021w (FAL1).

Yeast (Chichester, England) - 1 Feb 2000

Dardalhon M, Magaña-Schwencke N, Averbeck D

Abstract excerpt

The disruption of six novel yeast genes has been realized in two genetic backgrounds. Six open reading frames (ORFs) from chromosome IV, YDR013w, YDR014w, YDR015c, YDR018c, YDR020c and YDR021w, were disrupted using the KanMX4 marker and PCR-targeting with long flanking regions homologous (LFH) to the target locus. The deletants were verified at the molecular level, using PCR and Southern analysis. Sporulation and...

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PMID: 10649455
DOI: 10.1002/(SICI)1097-0061(200002)16:3<267::AID-YEA522>3.0.CO;2-N

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Source receipt: pubmed, retrieved 7 Sept 2026.

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