Article
Flanking direct repeats of hisG alter URA3 marker expression at the HWP1 locus of Candida albicans.
Microbiology (Reading, England) - 1 Apr 2005
Sharkey Laura L, Liao Wei-Li, Ghosh Anup K, Fonzi William A
Abstract excerpt
HWP1 encodes an adhesin of Candida albicans and has been implicated in filamentation and virulence. URA3, an often-used transformation selection marker, is apparently incorrectly expressed when integrated at the HWP1 locus, which results in an attenuated virulence phenotype. Expression of URA3 is compromised by ectopic integration at other loci as well. In contrast, prior studies from the authors' laboratory had...
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