The genetic interaction of kar2 and wbp1 mutations. Distinct functions of binding protein BiP and N-linked glycosylation in the processing pathway of secreted proteins in Saccharomyces cerevisiae.

Abstract excerpt

The endoplasmic binding protein BiP and N-linked glycosylation are proposed to be essential components in the processing pathway of secreted protein. In Saccharomyces cerevisiae, BiP is encoded by the KAR2 gene; WBP1 encodes an essential component of the N-oligosaccharyltransferase complex. wbp1 mutations result in reduced oligosaccharyltransferase activity and a temperature-sensitive phenotype. We show that a...

Topics

• Endoplasmic Reticulum
• Fungal Proteins
• Genes, Fungal
• Genotype
• Glycosylation
• HSP70 Heat-Shock Proteins
• Heat-Shock Proteins
• Hexosyltransferases
• Kinetics
• Membrane Proteins