Article

Constitutively-active Rheb mutants [T23M] and [E40K] drive increased production and secretion of recombinant protein in Chinese hamster ovary cells

2020-10-08

Abstract excerpt

Monoclonal antibodies are high value agents used for disease therapy (‘biologic drugs’) or as diagnostic tools which are widely used in the health care sector. They are generally manufactured in mammalian cells, in particular Chinese hamster ovary (CHO) cells cultured in defined media, and are harvested from the medium. Rheb is a small GTPase which, when bound to GTP, activates mechanistic target of rapamycin comp...

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Literature Corpus work: 38d4ada6-707f-589d-8f7d-c7cc03f1289e
DOI: 10.22541/au.160218204.42443382/v1

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