Article
Residues in the alphaH and alphaI helices of the HIV-1 reverse transcriptase thumb subdomain required for the specificity of RNase H-catalyzed removal of the polypurine tract primer.
The Journal of biological chemistry - 9 Jul 1999
Powell M D, Beard W A, Bebenek K, Howard K J, Le Grice S F, Darden T A, Kunkel T A, Wilson S H, Levin J G
Abstract excerpt
During retrovirus replication, reverse transcriptase (RT) must specifically interact with the polypurine tract (PPT) to generate and subsequently remove the RNA primer for plus-strand DNA synthesis. We have investigated the role that human immunodeficiency virus-1 RT residues in the alphaH and alphaI helices in the thumb subdomain play in specific RNase H cleavage at the 3'-end of the PPT; an in vitro assay...
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