Article
Alanine-scanning mutations in the "primer grip" of p66 HIV-1 reverse transcriptase result in selective loss of RNA priming activity.
The Journal of biological chemistry - 16 May 1997
Powell M D, Ghosh M, Jacques P S, Howard K J, Le Grice S F, Levin J G
Abstract excerpt
Alanine-scanning mutants of the primer grip region of human immunodeficiency virus type 1 reverse transcriptase were tested for their ability to extend RNA and DNA versions of the polypurine tract primer, and an oligonucleotide representing the 18-nucleotide sequence at the 3' end of tRNALys3. A...
Topics
- Alanine
- Amino Acid Sequence
- Gene Expression Regulation, Viral
- HIV Reverse Transcriptase
- HIV-1
- Humans
- Molecular Sequence Data
- Mutation
- RNA
- RNA, Viral