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Article

Mutations in the 5' end of the human immunodeficiency virus type 1 polypurine tract affect RNase H cleavage specificity and virus titer.

Journal of virology - 1 Oct 2003

McWilliams Mary Jane, Julias John G, Sarafianos Stefan G, Alvord W Gregory, Arnold Edward, Hughes Stephen H

Abstract excerpt

The RNase H activity of retroviral reverse transcriptases (RTs) degrades viral genomic RNA after it has been copied into DNA, removes the tRNA used to initiate negative-strand DNA synthesis, and generates and removes the polypurine tract (PPT) primer used to initiate positive-strand DNA synthesis. The cleavages that remove the tRNA and that generate and remove the PPT primer must be specific to generate linear...

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Identifiers

PMID: 14512562
DOI: 10.1128/jvi.77.20.11150-11157.2003
PMCID: PMC224987

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Source receipt: pubmed, retrieved 7 Sept 2026.

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