Article
Establishment of an activated macrophage cell line, A-THP-1, and its properties.
The Tohoku journal of experimental medicine - 1 Oct 1998
Tominaga T, Suzuki M, Saeki H, Matsuno S, Tachibana T, Kudo T
Abstract excerpt
A new macrophage cell line with activated character and unique morphology was isolated by selecting adherent cells from the human monocytic cell line THP-1. The original THP-1 cells had been cultured for more than 9 years using 25 cm2 flasks, when cells with a different morphology appeared, adhering to the bottoms of the culture flasks. These were selected by discarding floating nonadherent cells at every...
Topics
- Actins
- Aneuploidy
- Carboxylesterase
- Carboxylic Ester Hydrolases
- Cell Adhesion
- Cell Differentiation
- Cell Line
- Chromosomes, Human
- Cytokines
- HLA-DR Antigens
- Humans
- Isoenzymes
- Karyotyping
- L-Lactate Dehydrogenase
- Leukemia, Monocytic, Acute