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Conversion of a reporter gene for mitochondrial gene expression using iterative mega-prime PCR.

Gene - 16 Apr 1999

Chrzanowska-Lightowlers Z M, Temperley R J, McGregor A, Bindoff L A, Lightowlers R N

Abstract excerpt

Mammalian mitochondria possess their own multicopy genome, mtDNA. Although much is known about mtDNA replication and transcription, our knowledge of the mechanisms governing mt-RNA processing, stability and translation remains rudimentary. We have taken a step towards addressing these issues by altering the luciferase reporter gene to accommodate the variation in mitochondrial codon recognition. 19 essential...

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Identifiers

PMID: 10216263
DOI: 10.1016/s0378-1119(99)00082-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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