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Article

Quantitative assay system for specific enzyme activity using antibody: the case of protease, subtilisin BPN'.

Journal of biotechnology - 11 Dec 1998

Miyota Y, Komada S, Momose H, Taguchi S

Abstract excerpt

Assay on a high-quality microtiter plate was found to allow for quantitative analysis of bacterial serine protease, subtilisin BPN', and its mutant enzymes which had been genetically engineered to be adapted to low-temperatures (Taguchi et al., 1998. Appl. Environ. Microbiol. 64, 492-495), by using polyclonal antibody against subtilisin BPN'. The use of polyclonal antibody was crucial in normalizing the number of...

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Identifiers

PMID: 9866867
DOI: 10.1016/s0168-1656(98)00148-5

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