Quantitative assay system for specific enzyme activity using antibody: the case of protease, subtilisin BPN'.

doi:10.1016/s0168-1656(98)00148-5

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Article

Quantitative assay system for specific enzyme activity using antibody: the case of protease, subtilisin BPN'.

Journal of biotechnology - 11 Dec 1998

Miyota Y, Komada S, Momose H, Taguchi S

Abstract excerpt

Assay on a high-quality microtiter plate was found to allow for quantitative analysis of bacterial serine protease, subtilisin BPN', and its mutant enzymes which had been genetically engineered to be adapted to low-temperatures (Taguchi et al., 1998. Appl. Environ. Microbiol. 64, 492-495), by usi...

Topics

Animals
Antibodies
Bacillus subtilis
Biochemistry
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Mutation
Rabbits
Recombinant Proteins
Subtilisins

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