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Article

Stabilization of penicillin G acylase from Escherichia coli: site-directed mutagenesis of the protein surface to increase multipoint covalent attachment.

Applied and environmental microbiology - 1 Feb 2004

Abian Olga, Grazú Valeria, Hermoso Juan, González Ramón, García José Luis, Fernández-Lafuente Roberto, Guisán José Manuel

Abstract excerpt

Three mutations on the penicillin acylase surface (increasing the number of Lys in a defined area) were performed. They did not alter the enzyme's stability and kinetic properties; however, after immobilization on glyoxyl-agarose, the mutant enzyme showed improved stability under all tested condi...

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Identifiers

PMID: 14766616
DOI: 10.1128/AEM.70.2.1249-1251.2004
PMCID: PMC348938

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-14766616-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

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