Article
Immobilization of whole-cell penicillin G acylase by entrapping within polymethacrylamide beads.
Applied biochemistry and biotechnology - 1 Jan 2000
Hsiau L T, Lee W C, Wang F S
Abstract excerpt
Escherichia coli ATCC 11105 containing the periplasmic penicillin G acylase was entrapped within a copolymer of methacrylamide and N,N'-methylenebisacrylamide. A solution of monomer that was made up from methacrylamide and N,N'-methylenebisacrylamide dissolved in buffer was mixed with lyophilized cells and ammonium persulfate. This suspension was then pumped drop by drop into in soybean oil supplemented with...
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