Article
Generation of soluble and active subtilisin and alpha-chymotrypsin in organic solvents via hydrophobic ion pairing.
International journal of peptide and protein research - 1 Mar 1996
Meyer J D, Kendrick B S, Matsuura J E, Ruth J A, Bryan P N, Manning M C
Abstract excerpt
With very low concentrations of anionic detergents, such as sodium dodecyl sulfate (SDS) and Aerosol OT (AOT), it is possible to solubilize proteases in organic solvents, while retaining enzymatic activity. For example, the SDS-subtilisin BPN' complex catalyzes transesterification of Ac-Phe-OMe in ethanol with a kcat/Km of 36 M-1 s-1 for mutant M1 and 39 M-1 s-1 for the wild type. By comparison, M1 suspended in...
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