Article
Major DQ8-restricted T-cell epitopes for human GAD65 mapped using human CD4, DQA1*0301, DQB1*0302 transgenic IA(null) NOD mice.
Diabetes - 1 Mar 1999
Liu J, Purdy L E, Rabinovitch S, Jevnikar A M, Elliott J F
Abstract excerpt
The 65KD isoform of GAD is considered to be a major target autoantigen in many humans with autoimmune prediabetes or diabetes. The major histocompatibility complex class II allele DQA1*0301, DQB1*0302, which encodes HLA-DQ8, confers susceptibility to type 1 diabetes and occurs in up to 80% of affected individuals. To map T-cell epitopes for GAD65 restricted to the diabetes-associated DQ8 heterodimer, we generated...
Topics
- Amino Acid Sequence
- Animals
- Autoantibodies
- B-Lymphocytes
- CD4 Antigens
- Diabetes Mellitus, Type 1
- Epitopes
- Flow Cytometry
- Genetic Predisposition to Disease
- Glutamate Decarboxylase
- HLA-DQ Antigens