Article
Phenobarbital responsiveness conferred by the 5'-flanking region of the rat CYP2B2 gene in transgenic mice.
Gene - 4 Mar 1999
Ramsden R, Beck N B, Sommer K M, Omiecinski C J
Abstract excerpt
Phenobarbital (PB) is a prototype for a class of agents that produce marked transcriptional activation of a number of genes, including certain cytochrome P-450s. We used transgenic mouse approaches and multiple gene reporters to assess the functional consequences of specific deletions and site-specific mutations within the 2.5kb 5'-flanking region of the rat CYP2B2 gene. Protein-DNA interactions at the PBRU...
Topics
- Animals
- Aryl Hydrocarbon Hydroxylases
- Binding Sites
- CCAAT-Enhancer-Binding Proteins
- Chloramphenicol O-Acetyltransferase
- Cytochrome P-450 Enzyme System
- DNA
- DNA Footprinting
- DNA-Binding Proteins
- Deoxyribonuclease I
- Female
- Gene Expression Regulation
- Growth Hormone