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Comparative analysis of CRISPR off-target activity discovery tools following ex vivo editing of CD34 + hematopoietic stem and progenitor cells

2022-09-10

Abstract excerpt

While CRISPR-based editing most often occurs at DNA sequences with perfect homology to the guide RNA (gRNA), unintended editing can occur at highly homologous regions (i.e., off-target (OT) sites). Due to the pace at which genome editing therapies are approaching clinical applications, there is an emerging need to define effective workflows for investigating OT editing effects. A number of homology-dependent, in...

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Literature Corpus work: fdd861b7-0c66-5250-bd9d-d07e4b901335
DOI: 10.1101/2022.09.09.507306

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Comparative analysis of CRISPR off-target activity discovery tools following <i>ex vivo</i> editing of CD34 <sup>+</sup> hematopoietic stem and progenitor cells

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