Back to search

Article

Optimized guide RNA selection recommendations for using <i>sp</i> Cas9 gene editing in human hematopoietic stem and progenitor cells

2020-12-19

Abstract excerpt

Ribonucleoproteins (RNPs) are frequently applied for therapeutic gene editing as well as fundamental research, because the method is fast, viral free, and does not rely on clonal selection. We evaluated various parameters to genetically engineer human hematopoietic stem progenitor cells (HSPCs) using sp Cas9-RNPs and achieve gene editing efficiencies up to 80%. We find that single guide RNA (sgRNA) design is crit...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
983e88bf-07df-5016-8d23-4a85d1cd7b32
DOI
10.1101/2020.12.18.423329
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Optimized guide RNA selection recommendations for using <i>sp</i> Cas9 gene editing in human hematopoietic stem and progenitor cellsDOI 10.1101/2020.12.18.423329
Clinical hematopoietic stem cell-based gene therapySemantic proximity
Highly Efficient Genome Editing of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9Semantic proximity
Combined lentiviral- and RNA-mediated CRISPR/Cas9 delivery for efficient and traceable gene editing in human hematopoietic stem and progenitor cellsSemantic proximity
The changing landscape of gene editing in hematopoietic stem cells: a step towards Cas9 clinical translationSemantic proximity
Comparative analysis of CRISPR off-target activity discovery tools following <i>ex vivo</i> editing of CD34 <sup>+</sup> hematopoietic stem and progenitor cellsSemantic proximity
Near-perfect precise on-target editing of human hematopoietic stem and progenitor cellsSemantic proximity
Functional profiling of single CRISPR/Cas9-edited human long-term hematopoietic stem cellsSemantic proximity
Near-perfect precise on-target editing of human hematopoietic stem and progenitor cellsSemantic proximity
Functional Profiling of Single CRISPR/Cas9-Edited Human Long-Term Hematopoietic Stem CellsSemantic proximity
Gene editing without <i>ex vivo</i> culture evades genotoxicity in human hematopoietic stem cellsSemantic proximity
A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells.Semantic proximity
Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene TargetingSemantic proximity
Select a neighboring publication to make it the new centre.