Article

Validation and Long-Term Follow Up of CD33 Off-Targets Predicted In Vitro and In Silico Using Error-Corrected Sequencing in Rhesus Macaques

2020-07-05

Abstract excerpt

<h4>ABSTRACT</h4> The programmable nuclease technology CRISPR/Cas9 has revolutionized gene editing in the last decade. Due to the risk of off-target editing, accurate and sensitive methods for off-target characterization are crucial prior to applying CRISPR/Cas9 therapeutically. Here, we utilized a rhesus macaque model to ask whether CIRCLE-Seq (CS), an in vitro off-target prediction method, more accurately iden...

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Literature Corpus work: 49f76fb5-f323-58a6-b937-b9d0f7a4a9db
DOI: 10.1101/2020.07.05.186858

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Validation and Long-Term Follow Up of CD33 Off-Targets Predicted In Vitro and In Silico Using Error-Corrected Sequencing in Rhesus MacaquesDOI 10.1101/2020.07.05.186858Read

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