Article

CertPrime: a new oligonucleotide design tool for gene synthesis

2025-04-29

Abstract excerpt

The design of oligonucleotides with uniform hybridisation temperatures is essential for successful gene synthesis. However, current computational tools for oligonucleotide design face significant limitations, including difficulties in processing long DNA sequences, poor adaptability to specific experimen- tal conditions, limited control over oligonucleotide length, and challenges in minimising spurious dimer forma...

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Literature Corpus work: fb18ef3e-53d8-5ca3-b45a-d90d5f07edf6
DOI: 10.1101/2025.04.29.650686

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