Article
RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and mis-priming from structural variation.
BMC genomics - 3 Dec 2009
Piriyapongsa Jittima, Ngamphiw Chumpol, Assawamakin Anunchai, Wangkumhang Pongsakorn, Suwannasri Payiarat, Ruangrit Uttapong, Agavatpanitch Gallissara, Tongsima Sissades
Abstract excerpt
BACKGROUND: Polymerase chain reaction (PCR) is very useful in many areas of molecular biology research. It is commonly observed that PCR success is critically dependent on design of an effective primer pair. Current tools for primer design do not adequately address the problem of PCR failure due...
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